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Cell. 2001 Nov 16;107(4):451-64.

AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.

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  • 1Department of Pharmacology, Laboratory of Gene Regulation and Signal Transduction, University of California, San Diego, La Jolla, CA 92093, USA.

Abstract

Inherently unstable mammalian mRNAs contain AU-rich elements (AREs) within their 3' untranslated regions. Although found 15 years ago, the mechanism by which AREs dictate rapid mRNA decay is not clear. In yeast, 3'-to-5' mRNA degradation is mediated by the exosome, a multisubunit particle. We have purified and characterized the human exosome by mass spectrometry and found its composition to be similar to its yeast counterpart. Using a cell-free RNA decay system, we demonstrate that the mammalian exosome is required for rapid degradation of ARE-containing RNAs but not for poly(A) shortening. The mammalian exosome does not recognize ARE-containing RNAs on its own. ARE recognition requires certain ARE binding proteins that can interact with the exosome and recruit it to unstable RNAs, thereby promoting their rapid degradation.

PMID:
11719186
[PubMed - indexed for MEDLINE]
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