Binding of SLE autoantibodies to native poly(I), ROS-poly(I) and native DNA: a comparative study

J Autoimmun. 2001 Nov;17(3):199-205. doi: 10.1006/jaut.2001.0546.

Abstract

Reactive oxygen species (ROS) are implicated in a variety of human diseases. The formation of pathogenic anti-DNA antibodies in systemic lupus erythematosus (SLE) has been extensively investigated. ROS-modified DNA has been found to be a better antigen for anti-DNA antibodies found in SLE sera. A comparative binding of SLE autoantibodies with native poly(I), ROS-poly(I) and nDNA has been studied. Affinity-purified SLE IgG exhibited a high degree of specificity towards the ROS-modified poly(I) in comparison to native DNA and native poly(I), reiterated visually by gel retardation assay. The data suggested that hydroxyl radical-modified nucleic acids like RNA and DNA might be agent for the induction of circulating SLE anti-DNA autoantibodies.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Antinuclear / blood
  • Antibodies, Antinuclear / metabolism*
  • Autoantibodies / metabolism*
  • Binding Sites, Antibody*
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / metabolism
  • Lupus Erythematosus, Systemic / immunology*
  • Poly I / immunology*
  • Poly I / metabolism
  • Reactive Oxygen Species / immunology*
  • Reactive Oxygen Species / metabolism

Substances

  • Antibodies, Antinuclear
  • Autoantibodies
  • Immunoglobulin G
  • Reactive Oxygen Species
  • Poly I