Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Neuron. 2001 Oct 25;32(2):301-13.

Multivesicular release at climbing fiber-Purkinje cell synapses.

Author information

  • 1Vollum Institute, Oregon Health Sciences University L474, 3181 S. W. Sam Jackson Park Road, Portland, OR 97201, USA.

Abstract

Synapses driven by action potentials are thought to release transmitter in an all-or-none fashion; either one synaptic vesicle undergoes exocytosis, or there is no release. We have estimated the glutamate concentration transient at climbing fiber synapses on Purkinje cells by measuring the inhibition of excitatory postsynaptic currents (EPSCs) produced by a low-affinity competitive antagonist of AMPA receptors, gamma-DGG. The results, together with simulations using a kinetic model of the AMPA receptor, suggest that the peak glutamate concentration at this synapse is dependent on release probability but is not affected by pooling of transmitter released from neighboring synapses. We propose that the mechanism responsible for the elevated glutamate concentration at this synapse is the simultaneous release of multiple vesicles per site.

PMID:
11683999
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk