Abstract
Vascular endothelial growth factor B (VEGF-B), a member of the VEGF/PDGF family, is highly expressed in many tissues with two differentially spliced transcripts generating two secreted isoforms, VEGF-B167 and VEGF-B186. In this work, we have investigated the expression of VEGF-B in tissues and cell lines using techniques that can distinguish the two isoforms. The results showed that the VEGF-B167 isoform was predominantly expressed in most tissues, accounting for more than 80% of the total VEGF-B transcripts. The VEGF-B186 isoform was expressed at lower levels and only in a limited number of tissues. Moreover, the VEGF-B186 isoform was up-regulated in mouse and human tumor cell lines and primary tumors compared with their corresponding normal tissues. Taken together, our data suggest a fine genetic control of the expression of the two isoforms of VEGF-B, implying tissue- and cell-specific roles of the two VEGF-B isoforms.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Embryo, Mammalian / metabolism
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Endothelial Growth Factors / chemistry
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Endothelial Growth Factors / genetics
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Endothelial Growth Factors / metabolism*
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Endothelial Growth Factors / pharmacology
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Gene Expression Regulation
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Humans
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Immunoblotting
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Immunoglobulin G / immunology
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Lymphokines / genetics
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Lymphokines / pharmacology
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Melanoma / metabolism
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Mice
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Microscopy, Fluorescence
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Neoplasms / metabolism*
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Pheochromocytoma / metabolism
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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RNA Probes
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Recombinant Proteins / pharmacology
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Tissue Distribution
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Tumor Cells, Cultured
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor B
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Vascular Endothelial Growth Factors
Substances
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Endothelial Growth Factors
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Immunoglobulin G
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Lymphokines
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Protein Isoforms
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RNA Probes
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Recombinant Proteins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factor B
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Vascular Endothelial Growth Factors