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The amino acid sequence of a hypothalamic peptide, neurotensin.
The amino acid sequence of neurotensin, a hypotensive peptide isolated from acid-acetone extracts of bovine hypothalami, has been established as less than Glu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu-Oh. (The nomenclature and symbols follow the suggestions of the IUPAC-IUB Commission on Biochemical Nomenclature (1972) J. Biol. Chem. 247, 977). This was accomplished by sequence analyses performed on the intact peptide as well as its isolated tryptic, chymotryptic, and papain-generated fragments. The results of enzymic hydrolyses were consistent with the specificities of the enzymes used and indicated that all of the amino acids are unsubstituted and in the L configuration. The absence of non-amino acid constituents was further supported by analyses of electrophoretic mobility-molecular weight relationships of neurotensin and its fragments.
PMID: 1167549 [PubMed - indexed for MEDLINE]
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Cited by 12 PubMed Central articles
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PKD1, PKD2, and their substrate Kidins220 regulate neurotensin secretion in the BON human endocrine cell line.
Li J, Chen LA, Townsend CM Jr, Evers BM.
J Biol Chem. 2008 Feb 1; 283(5):2614-21. Epub 2007 Nov 29.
[J Biol Chem. 2008]
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Peptide nucleic acids targeted to the neurotensin receptor and administered i.p. cross the blood-brain barrier and specifically reduce gene expression.
Tyler BM, Jansen K, McCormick DJ, Douglas CL, Boules M, Stewart JA, Zhao L, Lacy B, Cusack B, Fauq A, et al.
Proc Natl Acad Sci U S A. 1999 Jun 8; 96(12):7053-8.
[Proc Natl Acad Sci U S A. 1999]
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Neurotensin-like immunoreactivity in the brain of the chicken, Gallus domesticus.
Esposito V, De Girolamo P, Gargiulo G.
J Anat. 1997 Nov; 191 ( Pt 4):537-46.
[J Anat. 1997]
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