Inhibitory effect of short-term bile duct ligation on hepatic cytochrome P450 of bile acid-depleted rats

Pathobiology. 2001;69(1):30-5. doi: 10.1159/000048755.

Abstract

In this study we analyzed the effect resulting from a short-term (1 h) bile duct obstruction in bile acid-depleted or taurocholate-replenished rats on liver cytochrome P450 enzyme system activity. Rats were depleted of endogenous bile acids and then subjected to a biliary obstruction for 1 h. Some of these depleted-obstructed rats were replenished previously to the obstruction with exogenous taurocholic acid (TC) and the others were treated with the solvent alone. To study the isolated effect of the bile acid, other rats were also previously depleted and then replenished with TC but they were obstructed briefly (20 min). CYP3A2-linked activity was evaluated in vivo with the aminopyrine breath test and in vitro by the measurement of nifedipine oxidase microsomal activity. The results suggested that bile flow suppression per se might inhibit this CYP-linked activity and that bile acid retention is not involved at least as a sole determinant.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopyrine
  • Animals
  • Bile Acids and Salts / analysis
  • Bile Acids and Salts / metabolism*
  • Bile Ducts / surgery
  • Cholestasis, Extrahepatic / metabolism*
  • Cytochrome P-450 CYP3A
  • Cytochrome P-450 Enzyme Inhibitors*
  • Cytochrome P-450 Enzyme System / analysis
  • Cytochrome P-450 Enzyme System / metabolism
  • Ligation
  • Liver / metabolism*
  • Male
  • Mixed Function Oxygenases / analysis
  • Rats
  • Rats, Wistar
  • Steroid Hydroxylases / metabolism
  • Time Factors

Substances

  • Bile Acids and Salts
  • Cytochrome P-450 Enzyme Inhibitors
  • Aminopyrine
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Steroid Hydroxylases
  • CYP3A protein, human
  • Cytochrome P-450 CYP3A
  • steroid hormone 6-beta-hydroxylase