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Parasitol Int. 2001 Sep;50(3):165-73.

Use of variability in the stage-specific transcription levels of Plasmodium falciparum in the selection of target genes.

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  • 1AstraZeneca R & D Bangalore, 277, T Chowdaiah Road, Malleswaram, Bangalore-560003, India. vidya.prasanna@astrazeneca.com


The malarial parasite Plasmodium falciparum exhibits several morphological and developmental stages. We have quantified the level of expression of a battery of genes in the ring and trophozoite stage-two of the most prominent stages in the erythrocytic development of the parasite. Using optimized RT-PCR, we observed that some of the genes show a large variation in stage-specific expression. We have also correlated the level of mRNA expression (of the target enzyme) to its metabolic requirement using specific inhibitors. This protocol gives us a handle to identify vulnerable target genes that could be used to develop antimalarials.

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