Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Parasitol Int. 2001 Sep;50(3):165-73.

Use of variability in the stage-specific transcription levels of Plasmodium falciparum in the selection of target genes.

Author information

  • 1AstraZeneca R & D Bangalore, 277, T Chowdaiah Road, Malleswaram, Bangalore-560003, India. vidya.prasanna@astrazeneca.com

Abstract

The malarial parasite Plasmodium falciparum exhibits several morphological and developmental stages. We have quantified the level of expression of a battery of genes in the ring and trophozoite stage-two of the most prominent stages in the erythrocytic development of the parasite. Using optimized RT-PCR, we observed that some of the genes show a large variation in stage-specific expression. We have also correlated the level of mRNA expression (of the target enzyme) to its metabolic requirement using specific inhibitors. This protocol gives us a handle to identify vulnerable target genes that could be used to develop antimalarials.

PMID:
11595573
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk