Porins from Salmonella enterica serovar Typhimurium induce TNF-alpha, IL-6 and IL-8 release by CD14-independent and CD11a/CD18-dependent mechanisms

Microbiology (Reading). 2001 Oct;147(Pt 10):2697-2704. doi: 10.1099/00221287-147-10-2697.

Abstract

Lipopolysaccharide (LPS) of Gram-negative bacteria and several surface components of Gram-positive bacteria utilize CD14 and CD11a/18 as cellular receptors to induce expression and release of cytokines. Of the surface components of Gram-negative bacteria, porins exhibit a biological activity similar to that of LPS. The results in this paper show that the mechanism of stimulation by porins of THP-1 cells enriched in CD14 receptor after treatment with 1,25-dihydroxyvitamin D(3) (vitamin D(3)) is independent of this receptor, but is partially dependent on CD11a/18 integrins.

MeSH terms

  • CD18 Antigens / metabolism*
  • Cell Line
  • Cytokines / metabolism*
  • Humans
  • Interleukin-1 / metabolism
  • Interleukin-8 / metabolism
  • Lipopolysaccharide Receptors / metabolism*
  • Lipopolysaccharides / immunology
  • Lymphocyte Function-Associated Antigen-1 / metabolism*
  • Monocytes / immunology
  • Porins / physiology*
  • Receptors, Cell Surface / metabolism
  • Salmonella typhimurium / immunology
  • Salmonella typhimurium / physiology*
  • Serotyping
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • CD18 Antigens
  • Cytokines
  • Interleukin-1
  • Interleukin-8
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • Lymphocyte Function-Associated Antigen-1
  • Porins
  • Receptors, Cell Surface
  • Tumor Necrosis Factor-alpha