Abstract
Mutations in the WT1 gene are found in a subset of Wilms' tumours and in certain other disorders such as Denys-Drash syndrome. The WT1 gene product is a zinc finger transcription factor for which many target genes have been suggested. Here we utilise gene targeting to generate cells containing only truncated forms of WT1, in which the DNA-binding region is disrupted. Examination of gene expression in these cells using cDNA macroarrays suggests two novel WT1 transcriptional targets, cyclin G1 (Ccng1), and insulin-like growth factor binding protein 4 (Igfbp4).
Copyright 2001 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / pharmacology
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Cell Line
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Cyclin G
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Cyclin G1
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Cyclins / genetics*
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Cyclins / metabolism
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Down-Regulation
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Gene Targeting / methods*
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Humans
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Insulin-Like Growth Factor Binding Protein 4 / genetics*
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Insulin-Like Growth Factor Binding Protein 4 / metabolism
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Oligonucleotide Array Sequence Analysis
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Tretinoin / pharmacology
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WT1 Proteins / genetics*
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WT1 Proteins / metabolism
Substances
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Antineoplastic Agents
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CCNG1 protein, human
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Cyclin G
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Cyclin G1
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Cyclins
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Insulin-Like Growth Factor Binding Protein 4
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WT1 Proteins
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Tretinoin