Truncation of WT1 results in downregulation of cyclin G1 and IGFBP-4 expression

Biochem Biophys Res Commun. 2001 Oct 5;287(4):977-82. doi: 10.1006/bbrc.2001.5693.

Abstract

Mutations in the WT1 gene are found in a subset of Wilms' tumours and in certain other disorders such as Denys-Drash syndrome. The WT1 gene product is a zinc finger transcription factor for which many target genes have been suggested. Here we utilise gene targeting to generate cells containing only truncated forms of WT1, in which the DNA-binding region is disrupted. Examination of gene expression in these cells using cDNA macroarrays suggests two novel WT1 transcriptional targets, cyclin G1 (Ccng1), and insulin-like growth factor binding protein 4 (Igfbp4).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology
  • Cell Line
  • Cyclin G
  • Cyclin G1
  • Cyclins / genetics*
  • Cyclins / metabolism
  • Down-Regulation
  • Gene Targeting / methods*
  • Humans
  • Insulin-Like Growth Factor Binding Protein 4 / genetics*
  • Insulin-Like Growth Factor Binding Protein 4 / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Tretinoin / pharmacology
  • WT1 Proteins / genetics*
  • WT1 Proteins / metabolism

Substances

  • Antineoplastic Agents
  • CCNG1 protein, human
  • Cyclin G
  • Cyclin G1
  • Cyclins
  • Insulin-Like Growth Factor Binding Protein 4
  • WT1 Proteins
  • Tretinoin