Biochim Biophys Acta. 2001 Sep 21;1520(3):187-94.

Characterization of NR13-related human cell death regulator, Boo/Diva, in normal and cancer tissues.

Lee R, Chen J, Matthews CP, McDougall JK, Neiman PE.

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Huntsman Cancer Institute and Section of Oncology, Department of Medicine, University of Utah, Salt Lake City, UT 84112, USA. ray.lee@hci.utah.edu

Abstract

Mouse Boo/Diva is an ovary-specific member of the Bcl-2 family identified through homology with the avian cell death antagonist NR13. We identified a human orthologue of Boo/Diva, which is highly conserved between mouse and human and related to avian NR13. Human Boo/Diva is also expressed in human liver and kidney in addition to the ovary. We found that green fluorescence protein (EGFP)-tagged Boo/Diva was not exclusively localized to mitochondria before the induction of apoptosis. However, EGFP-Boo/Diva translocated to mitochondria in the process of apoptosis induced by vincristine, a microtubule-interfering agent. Overexpression of human Boo/Diva promoted cell death in HeLa and 293 cells. The cell death antagonist Bcl-XL interacts with Boo, but is unable to protect 293 cells from Boo/Diva-induced cell death. Finally, we mapped human Boo/Diva to chromosome 15q21, a locus known to be related to human cervical cancer. Moreover, we found that genomic DNAs of three of 24 human cervical cancer samples display deletions within their Boo/Diva genes. This result suggests a role for human Boo/Diva in the pathogenesis of cervical cancer.

PMID:: 11566354; [PubMed - indexed for MEDLINE]

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