Abstract
Calli were induced either from excised rice embryos or from whole seeds in the presence of 1 to 5 mg l-1 NAA. After 12 days of culture, calli were induced only from excised rice embryos. We found that excised embryos accumulated NAA up to 6 times higher concentration than did whole seeds. In the presence of 1 to 5 mg l-1 NAA and 2 to 10 mg l-1 kinetin, chlorophyllous calli were induced from excised rice embryos. Chlorophyll contents in the callus tissue increased with increasing kinetin concentration while percent callus induction decreased. The total chlorophyll content was linearly correlated with the ratio of kinetin to NAA in the medium.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division / physiology
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Cells, Cultured
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Chlorophyll / biosynthesis
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Chromatography, Thin Layer
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Culture Media, Conditioned / analysis
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Culture Media, Conditioned / pharmacology
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Kinetin / pharmacokinetics*
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Naphthaleneacetic Acids / pharmacokinetics*
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Oryza / drug effects
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Oryza / growth & development
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Oryza / physiology*
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Plant Growth Regulators / pharmacokinetics*
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Seeds / cytology
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Seeds / drug effects
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Seeds / growth & development*
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Time Factors
Substances
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Culture Media, Conditioned
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Naphthaleneacetic Acids
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Plant Growth Regulators
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Chlorophyll
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1-naphthaleneacetic acid
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Kinetin