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Curr Genet. 2001 Jul;39(5-6):388-93.

Efficient Agrobacterium tumefaciens-mediated gene disruption in the phytopathogen Mycosphaerella graminicola.

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  • 1Laboratory of Phytopathology, Wageningen University, The Netherlands.


Agrobacterium tumefaciens-mediated transformation has been successfully applied to the wheat pathogen Mycosphaerella graminicola. Both protoplasts and intact cells have been transformed to hygromycin B resistance. Furthermore, A. tumefaciens-mediated transformation using homologous DNA originating from the M. graminicola ABC transporter gene MgAtr2 resulted in the efficient generation of disruption mutants. In 44% of the transformants, disruption of MgAtr2 was achieved and transformants resulted from the integration of a single copy of the transforming DNA. These results indicate that A. tumefaciens-mediated transformation is a useful tool to generate targeted gene disruption in the phytopathogen M. graminicola, where gene targeting by conventional methods is hardly possible.

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