A highly specific one-step PCR - assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis

T Arnold; A Hensel; R Hagen; S Aleksic; H Neubauer; H C Scholz

doi:10.1078/0723-2020-00040

A highly specific one-step PCR - assay for the rapid discrimination of enteropathogenic Yersinia enterocolitica from pathogenic Yersinia pseudotuberculosis and Yersinia pestis

Syst Appl Microbiol. 2001 Jul;24(2):285-9. doi: 10.1078/0723-2020-00040.

Authors

T Arnold¹, A Hensel, R Hagen, S Aleksic, H Neubauer, H C Scholz

Affiliation

¹ Institute of Animal Hygiene and Public Veterinary Health, Leipzig, Germany.

PMID: 11518333
DOI: 10.1078/0723-2020-00040

Abstract

Based on differences within the yopT-coding region of Yersinia. enterocolitica, Y pseudotuberculosis and Y pestis, a rapid and sensitive one-step polymerase chain reaction assay with high specificity for pathogenic Y enterocolitica was developed. By this method pathogenic isolates of Y enterocolitica can be easily identified and discriminated from other members of this genus. The entire coding sequence of the yopT effector gene of Y. pseudotuberculosis Y36 was determined.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics*
Base Sequence
Cysteine Endopeptidases
Cytotoxins*
Feces / microbiology
Guinea Pigs
Humans
Molecular Sequence Data
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Sequence Analysis, DNA
Time Factors
Yersinia Infections / microbiology*
Yersinia enterocolitica / classification*
Yersinia enterocolitica / genetics
Yersinia enterocolitica / pathogenicity
Yersinia pestis / classification*
Yersinia pestis / genetics
Yersinia pestis / pathogenicity
Yersinia pseudotuberculosis / classification*
Yersinia pseudotuberculosis / genetics
Yersinia pseudotuberculosis / pathogenicity

Substances

Bacterial Proteins
Cytotoxins
YopT protein, Yersinia
Cysteine Endopeptidases