Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Parasitol Res. 2001 Jul;87(7):556-8.

Increasing the sensitivity of PCR detection in bovine preputial smegma spiked with Tritrichomonas foetus by the addition of agar and resin.

Author information

  • 1Department of Parasitology, The First Military Medical University, Guangzhou, China. xgchen@fjnet.guangzhou.gd.cn

Abstract

Methods for the extraction of DNA from the preputial smegma of cattle infected with Tritrichomonas foetus for the purposes of polymerase chain reaction (PCR) detection are usually time-consuming, relatively insensitive and require hazardous chemicals. In order to solve these problems, we have developed a rapid, sensitive and harmless method to extract quality DNA from preputial smegma spiked with T. foetus. Results indicate that the addition of 5% Chelex-100 resin and 0.05% agar solution to the spiked smegma before the process of DNA extraction by the boiling method can significantly increase the sensitivity of PCR detection. This improved method may be suitable for routine DNA extraction for the diagnosis of cattle and even human trichomoniasis by PCR.

PMID:
11484853
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk