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Zhonghua Jie He He Hu Xi Za Zhi. 1998 Dec;21(12):727-9.

[Study on the mutations of ahpC genes in M. tuberculosis isoniazid-resistant isolates].

[Article in Chinese]

Author information

  • 1Tuberculosis Research Laboratory, 309th Hospital, PLA, Beijing 100091.

Abstract

OBJECTIVE:

To investigate the mutations of ahpC genes in M. tuberculosis isoniazid-resistant isolates, and to study the correlation between ahpC alterations and isoniazid resistance.

METHOD:

Analyzing the ahpC coding sequences and ahpC promoters in 62 M. tuberculosis clinical isolates with PCR-SSCP method. M. tuberculosis strain H37RV was used as control.

RESULT:

The ahpC coding sequences showed normal SSCP profiles in 62 clinical isolates. AhpC promoter mutations were identified in 5 of 12 high isoniazid-resistant strains, and were not altered in 18 low isoniazid-resistant and 32 drug-sensitive isolates.

CONCLUSION:

The mutation of ahpC coding gene was not correlated with isoniazid-resistant M. tuberculosis isolates. AhpC promoter mutation was a compensatory change in katG-defective and catalase-negative isolates, may be used as a indirect marker for high resistance to isoniazid.

PMID:
11480074
[PubMed - indexed for MEDLINE]
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