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Med Pediatr Oncol. 2001 Jan;36(1):37-41.

Detailed molecular analysis of 1p36 in neuroblastoma.

Author information

  • 1Division of Oncology, Children's Hospital of Philadelphia, Pennsylvania 19104-4318, USA. white@genome.chop.edu

Abstract

BACKGROUND:

Several lines of evidence es tablish that chromosome band 1p36 is frequently deleted in neuroblastoma primary tumors and cell lines, suggesting that a tumor suppressor gene within this region is involved in the development of this tumor.

PROCEDURE:

We analyzed the status of 1p36 in primary neuroblastomas and cell lines to define the region of consistent rearrangement.

RESULTS:

Loss of heterozygosity (LOH) studies of primary neuro blastomas identified allelic loss in 135 of 503 tumors (27%), with the smallest region of overlap (SRO) defined distal to D15214 (1p36.3). No homozygous deletions were detected at 120 loci mapping to 1p36.1-p36.3 in a panel of 46 neuroblastoma cell lines. A recently identified patient with neuroblastoma was found to have a constitutional deletion within 1p36.2-p36.3, and this deletion, when combined with the LOH results, defined a smaller SRO of one megabase within 1p36.3. We constructed a comprehensive integrated map of chromosome 1 containing 11,000 markers and large-insert clones, a high-resolution radiation hybrid (RH) map of 1p36, and a P1-artificial chromosome (PAC) contig spanning the SRO, to further characterize the region of interest. Over 768 kb (75%) of the SRO has been sequenced to completion. Further analysis of distal 1p identified 113 transcripts localizing to 1p36, 21 of which were mapped within the SRO.

CONCLUSION:

This analysis will identify suitable positional candidate transcripts for mutational screening and subsequent identification of the 1p36.3 neuroblastoma suppressor gene.

PMID:
11464901
[PubMed - indexed for MEDLINE]
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