Cell. 2001 Jul 13;106(1):23-34.

Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.

Grishok A, Pasquinelli AE, Conte D, Li N, Parrish S, Ha I, Baillie DL, Fire A, Ruvkun G, Mello CC.

Source

Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.

Abstract

RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation.

PMID:: 11461699; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Miscellaneous

See the articles recommended by F1000Prime's Faculty of 5,000 renowned researchers and clinicians, assisted by 5,000 associates. - F1000

Supplemental Content

Save items

Related citations in PubMed

Review Control of developmental timing by small temporal RNAs: a paradigm for RNA-mediated regulation of gene expression. [Bioessays. 2002]
Review Control of developmental timing by small temporal RNAs: a paradigm for RNA-mediated regulation of gene expression.
Banerjee D, Slack F. Bioessays. 2002 Feb; 24(2):119-29.
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. [Nature. 2000]
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.
Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR, Ruvkun G. Nature. 2000 Feb 24; 403(6772):901-6.
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans. [Genes Dev. 2001]
Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C. elegans.
Ketting RF, Fischer SE, Bernstein E, Sijen T, Hannon GJ, Plasterk RH. Genes Dev. 2001 Oct 15; 15(20):2654-9.
Large-scale RNAi screens identify novel genes that interact with the C. elegans retinoblastoma pathway as well as splicing-related components with synMuv B activity. [BMC Dev Biol. 2007]
Large-scale RNAi screens identify novel genes that interact with the C. elegans retinoblastoma pathway as well as splicing-related components with synMuv B activity.
Ceron J, Rual JF, Chandra A, Dupuy D, Vidal M, van den Heuvel S. BMC Dev Biol. 2007 Apr 6; 7:30. Epub 2007 Apr 6.
Review Control of developmental timing in Caenorhabditis elegans. [Curr Opin Genet Dev. 2000]
Review Control of developmental timing in Caenorhabditis elegans.
Ambros V. Curr Opin Genet Dev. 2000 Aug; 10(4):428-33.

See reviews... See all...

Cited by over 100 PubMed Central articles

Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. [PLoS One. 2013]
Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.
Kasim V, Wu S, Taira K, Miyagishi M. PLoS One. 2013; 8(3):e59445. Epub 2013 Mar 19.
Pax6 regulates gene expression in the vertebrate lens through miR-204. [PLoS Genet. 2013]
Pax6 regulates gene expression in the vertebrate lens through miR-204.
Shaham O, Gueta K, Mor E, Oren-Giladi P, Grinberg D, Xie Q, Cvekl A, Shomron N, Davis N, Keydar-Prizant M, et al. PLoS Genet. 2013 Mar; 9(3):e1003357. Epub 2013 Mar 14.
The draft genome and transcriptome of Panagrellus redivivus are shaped by the harsh demands of a free-living lifestyle. [Genetics. 2013]
The draft genome and transcriptome of Panagrellus redivivus are shaped by the harsh demands of a free-living lifestyle.
Srinivasan J, Dillman AR, Macchietto MG, Heikkinen L, Lakso M, Fracchia KM, Antoshechkin I, Mortazavi A, Wong G, Sternberg PW. Genetics. 2013 Apr; 193(4):1279-95. Epub 2013 Feb 14.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Genes and mechanisms related to RNA interference regulate expression of the smal...
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.
Cell. 2001 Jul 13 ;106(1):23-34.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Miscellaneous

Supplemental Content

Save items

Related citations in PubMed

Cited by over 100 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information