Visualization of biallelic expression of the imprinted SNRPN gene induced by inhibitors of DNA methylation and histone deacetylation

Biosci Biotechnol Biochem. 2001 May;65(5):1236-9. doi: 10.1271/bbb.65.1236.

Abstract

We have adapted a fluorescence in situ hybridization (FISH) method to detect nascent RNA molecules of the imprinted SNRPN gene at the initial transcription sites in nuclei of human HL60 and WI38 cells. Simultaneous detection of RNA and DNA of SNRPN by FISH using the cosmid probe confirmed its monoallelic expression in these cell lines. Treatment of both cells by inhibitors of DNA methylation and histone deacetylation resulted in time-dependent increase of the cell population with the biallelic expression of SNRPN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Alleles*
  • Autoantigens / genetics*
  • DNA Methylation*
  • Gene Expression Regulation / drug effects*
  • Genomic Imprinting*
  • HL-60 Cells
  • Histone Deacetylases / metabolism
  • Histones / metabolism
  • Humans
  • In Situ Hybridization, Fluorescence
  • Ribonucleoproteins, Small Nuclear*
  • snRNP Core Proteins

Substances

  • Autoantigens
  • Histones
  • Ribonucleoproteins, Small Nuclear
  • SNRPN protein, human
  • snRNP Core Proteins
  • Histone Deacetylases