Biosci Biotechnol Biochem. 2001 May;65(5):1230-5.

gsk disruption leads to guanosine accumulation in Escherichia coli.

Matsui H, Shimaoka M, Takenaka Y, Kawasaki H, Kurahashi O.

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Fermentation & Biotechnology Laboratories, Ajinomoto Co., Inc., Kawasaki-shi, Kanagawa, Japan. hiroshimatsui@ajinomoto.com

Abstract

We tried some improvement of inosine production using an inosine-producing mutant of Escherichia coli which is deficient in purF (phosphoribosylpyrophosphate (PRPP) amidotransferase gene), purA (succinyl-adenosine 5'-monophosphate (AMP) synthetase gene), deoD (purine nucleoside phosphorylase gene), purR (purine repressor gene) and add (adenosine deaminase gene), and harboring the desensitized PRPP amidotransferase gene as a plasmid. The guaB (inosine 5'-monophosphate (IMP) dehydrogenase gene) disruption brought about a slightly positive effect on the inosine productivity. Alternatively, the gsk (guanosine-inosine kinase gene) disruption caused a considerable amount of guanosine accumulation together with a slight increase in the inosine productivity. The further addition of guaC (guanosine 5'-monophosphate (GMP) reductase gene) disruption did not lead to an increased guanosine accumulation, but brought about the decrease of inosine accumulation.

PMID:: 11440147; [PubMed - indexed for MEDLINE]

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