Amino acid sequence alignment of selected group I to IV defensins. Rs-AFP 1 to 4 are from Raphanus sativas (radish, accession no. P30225, P30230, 024332, and 024331), At-AFP1 to 3 are from Arabidopsis thaliana (thale cress, accession no. P30224, 080995, and 080994), AFP2-BRANA (rape, accession no. P30226) and AFP3-BRANA (rape, accession no. Q39313) are from Brassica napus, AFP1-BRARA (accession no. P30227) and AFP2-BRARA (accession no. P30228) are from B. rapa, AFP1_SINAL (white mustard, accession no. P30231) and AF2A_SINAL (white mustard, accession no. P30232) are from Sinapis alba, Ct-AMP1 (accession no. S66219) is from Clitoria ternate, Ah-AMP1 (common horse chestnut, accession no. S66218) is from Aesculus hippocastanum, Dm-AMP1 (bedding dahlia seeds, accession no. AAB34972) is from Dahlia merckii, St-PTH1 (potato tubers, accession no. AAB 31351) is from Solanum tuberosum, Siα2 (sorghum, accession no. P21924) is from Sorghum bicolor, and So-D1- to -7 (spinach) are from Spinacia oleracea. Alignments were done with the ClustalW program;∗ indicates amino acid identity.