Detection of antibodies to the nonstructural protein (NS1) of influenza A virus allows distinction between vaccinated and infected horses

Vet Microbiol. 2001 Sep 20;82(2):111-9. doi: 10.1016/s0378-1135(01)00366-2.

Abstract

Antibodies to the nonstructural protein (NS1) of A/equine/Miami/1/63 (H3N8) influenza virus were detected exclusively in the sera of mice experimentally infected with A/Aichi/2/68 (H3N2) and horses infected with A/equine/Kentucky/1/81 (H3N8) or A/equine/La Plata/1/93 (H3N8), but not in those of the animals immunized with the inactivated viruses, by enzyme-linked immunosorbent assay (ELISA) using a recombinant NS1 as antigen. The results indicate that the present method is useful for serological diagnosis to distinguish horses infected with equine H3 influenza viruses from those immunized with the inactivated vaccine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis*
  • Female
  • Horse Diseases / diagnosis*
  • Horse Diseases / immunology
  • Horse Diseases / prevention & control
  • Horses
  • Influenza A virus / immunology*
  • Influenza Vaccines / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Orthomyxoviridae Infections / diagnosis
  • Orthomyxoviridae Infections / immunology
  • Orthomyxoviridae Infections / veterinary*
  • Vaccination / veterinary*
  • Vaccines, Inactivated
  • Viral Nonstructural Proteins / immunology*

Substances

  • Antibodies, Viral
  • Influenza Vaccines
  • Vaccines, Inactivated
  • Viral Nonstructural Proteins