Chem Senses. 2001 Jun;26(5):529-31.

Detection and removal of an artefact fatty acid from the binding site of recombinant Bombyx mori pheromone-binding protein.

Oldham NJ, Krieger J, Breer H, Svatos A.

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Max-Planck-Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany. oldham@ice.mpg.de

Abstract

Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coli expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described to overcome what would appear to be a general problem with recombinant lepidopteran PBPs.

PMID:: 11418499; [PubMed - indexed for MEDLINE]

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