Conversion of cyclic nonaketides to lovastatin and compactin by a lovC deficient mutant of Aspergillus terreus

K Auclair; J Kennedy; C R Hutchinson; J C Vederas

doi:10.1016/s0960-894x(01)00290-6

Conversion of cyclic nonaketides to lovastatin and compactin by a lovC deficient mutant of Aspergillus terreus

Bioorg Med Chem Lett. 2001 Jun 18;11(12):1527-31. doi: 10.1016/s0960-894x(01)00290-6.

Authors

K Auclair¹, J Kennedy, C R Hutchinson, J C Vederas

Affiliation

¹ Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2.

PMID: 11412974
DOI: 10.1016/s0960-894x(01)00290-6

Abstract

Investigation of the post-PKS biosynthetic steps to the cholesterol-lowering agent lovastatin (1) using an Aspergillus terreus strain with a disrupted lovC gene, which is essential for formation of 4a,5-dihydromonacolin L (3), shows that 7 and 3 are precursors to 1, and demonstrates that lovastatin diketide synthase (lovF protein) does not require lovC.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Anticholesteremic Agents / metabolism
Aspergillus / enzymology*
Aspergillus / genetics
Aspergillus / metabolism
Fungal Proteins*
Hydroxymethylglutaryl-CoA Reductase Inhibitors / metabolism
Lovastatin / analogs & derivatives*
Lovastatin / biosynthesis*
Multienzyme Complexes / genetics*
Multienzyme Complexes / metabolism

Substances

Anticholesteremic Agents
Fungal Proteins
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Multienzyme Complexes
lovC protein, Aspergillus terreus
mevastatin
Lovastatin