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Methods. 2001 Jul;24(3):297-306.

High-throughput yeast two-hybrid assays for large-scale protein interaction mapping.

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  • 1Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.


Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system.

Copyright 2001 Academic Press.

[PubMed - indexed for MEDLINE]
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