Your browser version may not work well with NCBI's Web applications. More information here...
1: Am J Hematol. 2001 Jul;67(3):189-93.Links

Molecular characterization of hemoglobin C in Thailand.

Sanchaisuriya K, Fucharoen G, Sae-ung N, Siriratmanawong N, Surapot S, Fucharoen S.

Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.

We describe hematologic and DNA characterization of 12 hemoglobin C heterozygotes and three compound heterozygotes for hemoglobin C and hemoglobin E found in Thailand. Amplification and DNA analysis of genomic DNA by the polymerase chain reaction procedure permitted the identification of the beta(C) mutation at codon 6 of beta-globin gene (beta 6; GAG-AAG). beta-Globin gene haplotype analysis demonstrated that all beta(C) globin genes detected in these Thai individuals were associated with the haplotype (+ - - - - - +), indicating a non-African origin of this abnormal hemoglobin in Thailand. On routine hemoglobin typing, hemoglobin C is usually mistakenly identified as hemoglobin E because of theirs similar mobilities on cellulose acetate electrophoresis. The simple DNA assay for hemoglobin C based on an allele-specific polymerase chain reaction for accurate diagnosis of hemoglobin C was therefore developed. Copyright 2001 Wiley-Liss, Inc.

PMID: 11391717 [PubMed - indexed for MEDLINE]