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J Biol Chem. 2001 Jul 27;276(30):28014-21. Epub 2001 May 29.

Purification and characterization of native conventional kinesin, HSET, and CENP-E from mitotic hela cells.

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  • 1Department of Molecular, Cellular, and Developmental Biology and the Materials Research Laboratory, University of California, Santa Barbara, California 93106, USA.

Abstract

We have developed a strategy for the purification of native microtubule motor proteins from mitotic HeLa cells and describe here the purification and characterization of human conventional kinesin and two human kinesin-related proteins, HSET and CENP-E. We found that the 120-kDa HeLa cell conventional kinesin is an active motor that induces microtubule gliding at approximately 30 microm/min at room temperature. This active form of HeLa cell kinesin does not contain light chains, although light chains were detected in other fractions. HSET, a member of the C-terminal kinesin subfamily, was also purified in native form for the first time, and the protein migrates as a single band at approximately 75 kDa. The purified HSET is an active motor that induces microtubule gliding at a rate of approximately 5 microm/min, and microtubules glide for an average of 3 microm before ceasing movement. Finally, we purified native CENP-E, a kinesin-related protein that has been implicated in chromosome congression during mitosis, and we found that this form of CENP-E does not induce microtubule gliding but is able to bind to microtubules.

PMID:
11382767
[PubMed - indexed for MEDLINE]
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