For meeting the clinic needs in simultaneous pancreas and kidney transplantation (SPK), we successfully establish a syngeneic SPK transplatation model in Lewis rats. The results indicate that this model is feasible with a 82.6% successful rate of operation and a 69.6% survival rate in the first postoperative week. In long-term survived rats, the blood supplies are well established, function of the grafts (pancreas and kidney) maintains normal. This model is suitable for theoretical research in SPK transplantation for its reasonable physiology with pancreatic juice drained into intestine and reduced postoperative complications in urinary tract and carbohydrate metabolism.