Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Science. 2001 May 18;292(5520):1385-8.

Genetic analysis of digestive physiology using fluorescent phospholipid reporters.

Author information

  • 1Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210, USA. sfarber@lac.jci.tju.edu

Abstract

Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

PMID:
11359013
[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk