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    J Bacteriol. 2001 Jun;183(11):3276-81.

    Characterization of a three-component vanillate O-demethylase from Moorella thermoacetica.

    Source

    Department of Biochemistry, Beadle Center, University of Nebraska-Lincoln, Lincoln, NE 68588-0664, USA.

    Abstract

    The Moorella thermoacetica aromatic O-demethylase was characterized as an inducible three-component system with similarity to the methanogenic methanol, methylamine, and methanethiol methyltransferases and to the O-demethylase system from Acetobacterium dehalogenans. MtvB catalyzes methyl transfer from a phenylmethylether to the cobalt center of MtvC, a corrinoid protein. MtvA catalyzes transmethylation from MtvC to tetrahydrofolate, forming methyltetrahydrofolate. Cobalamin can substitute for MtvC.

    PMID:
    11344134
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC99624
    Free PMC Article

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