Display Settings:

Format

Send to:

Choose Destination
    Exp Cell Res. 2001 May 15;266(1):95-105.

    Cripto-1 enhances migration and branching morphogenesis of mouse mammary epithelial cells.

    Source

    Laboratory of Tumor Immunology and Biology, Tumor Growth Factor Section, National Cancer Institute, National Institutes of Health, Building 10, Room 5B39, 10 Center Drive, Bethesda, Maryland 20892, USA.

    Abstract

    Cripto-1 is an EGF-CFC protein that performs an important role during early vertebrate development and is overexpressed in several types of human cancer. In the present study mouse EpH4, NMuMG, and TAC-2 mammary epithelial cells that are negative for endogenous cripto-1 expression were transfected with the murine cripto-1 cDNA. Cripto-1-transfected cell lines exhibited functional and physiological differences from the original cell lines including enhanced anchorage-independent growth in soft agar (EpH4 cells), growth in serum-free medium, increased proliferation, and formation of branching, duct-like structures when grown in a three-dimensional collagen type I matrix. Furthermore, cripto-1-expressing cell lines showed elevated migration in vitro in Boyden chamber and wound-healing assays. These results indicate that cripto-1 can function through an autocrine pathway that enables mammary epithelial cells to undergo an epithelial to mesenchymal transition.

    PMID:
    11339828
    [PubMed - indexed for MEDLINE]

      Supplemental Content

      Icon for Elsevier Science

      Save items

      loading

      Recent activity

      Your browsing activity is empty.

      Activity recording is turned off.

      Turn recording back on

      See more...
      Write to the Help Desk