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Science. 2001 May 18;292(5520):1382-5. Epub 2001 May 3.

Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome.

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  • 1Department of Biology, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, CA 91125, USA.

Abstract

SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits--including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export--underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.

PMID:
11337588
[PubMed - indexed for MEDLINE]
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