Biotechniques. 2001 Apr;30(4):816-20, 822-6, 828 passim.

Genetic transformation of yeast.

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Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Ave., Winnipeg, MB, Canada, R3M 0W3. gietz@cc.umanitoba.ca

Abstract

Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S. cerevisiae to other important yeast species is discussed. The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.

PMID:: 11314265; [PubMed - indexed for MEDLINE]

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