Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 2001 May;21(10):3589-97.

Protein interactions of the MLL PHD fingers modulate MLL target gene regulation in human cells.

Author information

  • 1Cancer Center, Medical Center, Loyola University-Chicago, Maywood, Illinois 60153.

Abstract

The PHD fingers of the human MLL and Drosophila trx proteins have strong amino acid sequence conservation but their function is unknown. We have determined that these fingers mediate homodimerization and binding of MLL to Cyp33, a nuclear cyclophilin. These two proteins interact in vitro and in vivo in mammalian cells and colocalize at specific nuclear subdomains. Overexpression of the Cyp33 protein in leukemia cells results in altered expression of HOX genes that are targets for regulation by MLL. These alterations are suppressed by cyclosporine and are not observed in cell lines that express a mutant MLL protein without PHD fingers. These results suggest that binding of Cyp33 to MLL modulates its effects on the expression of target genes.

PMID:
11313484
[PubMed - indexed for MEDLINE]
PMCID:
PMC100280
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk