Abstract
A primary mechanism of lentivirus persistence is the ability of these viruses to evolve in response to biological and immunological selective pressures with a remarkable array of genetic and antigenic variations that constitute a perpetual natural experiment in genetic engineering. A widely accepted paradigm of lentivirus evolution is that the rate of genetic variation is correlated directly with the levels of virus replication: the greater the viral replication, the more opportunities that exist for genetic modifications and selection of viral variants. To test this hypothesis directly, we examined the patterns of equine infectious anemia virus (EIAV) envelope variation during a 2.5-year period in experimentally infected ponies that differed markedly in clinical progression and in steady-state levels of viral replication as indicated by plasma virus genomic RNA assays. The results of these comprehensive studies revealed for the first time similar extents of envelope gp90 variation in persistently infected ponies regardless of the number of disease cycles (one to six) and viremia during chronic disease. The extent of envelope variation was also independent of the apparent steady-state levels of virus replication during long-term asymptomatic infection, varying from undetectable to 10(5) genomic RNA copies per ml of plasma. In addition, the data confirmed the evolution of distinct virus populations (genomic quasispecies) associated with sequential febrile episodes during acute and chronic EIA and demonstrated for the first time ongoing envelope variation during long-term asymptomatic infections. Finally, comparison of the rates of evolution of the previously defined EIAV gp90 variable domains demonstrated distinct differences in the rates of nucleotide and amino acid sequence variation, presumably reflecting differences in the ability of different envelope domains to respond to immune or other biological selection pressures. Thus, these data suggest that EIAV variation can be associated predominantly with ongoing low levels of virus replication and selection in target tissues, even in the absence of substantial levels of plasma viremia, and that envelope variation continues during all stages of persistent infection as the virus successfully avoids clearance by host defense mechanisms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute Disease
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Amino Acid Sequence
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Animals
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Base Sequence
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Chronic Disease
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DNA, Viral
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Disease Progression
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Equine Infectious Anemia / physiopathology
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Equine Infectious Anemia / virology
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Evolution, Molecular*
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Genetic Variation
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Genome, Viral*
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Glycoproteins / classification
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Glycoproteins / genetics*
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Horses
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Infectious Anemia Virus, Equine / classification
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Infectious Anemia Virus, Equine / genetics*
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Molecular Sequence Data
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Phylogeny
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Sequence Homology, Amino Acid
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Time Factors
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Viral Envelope Proteins / classification
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Viral Envelope Proteins / genetics*
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Viremia / virology
Substances
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DNA, Viral
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Glycoproteins
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SU protein, equine infectious anemia virus
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Viral Envelope Proteins
Associated data
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GENBANK/AF298671
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