Genome-wide analysis of protein-DNA interactions in living cells

B F Pugh; D S Gilmour

doi:10.1186/gb-2001-2-4-reviews1013

Genome-wide analysis of protein-DNA interactions in living cells

Genome Biol. 2001;2(4):REVIEWS1013. doi: 10.1186/gb-2001-2-4-reviews1013. Epub 2001 Apr 4.

Authors

B F Pugh¹, D S Gilmour

Affiliation

¹ Center for Gene Regulation, Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.

Abstract

Understanding the regulation of gene expression requires an analysis of gene-specific transcription factors. This review highlights recent work that uses protein-DNA crosslinking, immunoprecipitation and DNA microarrays to determine the binding sites for specific transcription factors throughout the yeast genome.

Publication types

Review

MeSH terms

Binding Sites / genetics
DNA, Fungal / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Genome, Fungal*
Protein Binding
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism

Substances

DNA, Fungal
DNA-Binding Proteins
Transcription Factors