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Biosci Biotechnol Biochem. 2001 Feb;65(2):438-41.

Production of galactooligosaccharides from lactose using a beta-glucosidase from Thermus sp. Z-1.

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  • 1Graduate School of Practical Sciences, Showa Women's University, Tokyo, Japan.


A thermostable beta-glucosidase from Thermus sp. Z-1 that not only hydrolyzes beta-glucosides but also beta-galactosides was shown to efficiently produce oligosaccharides during hydrolysis of lactose. The yield of oligosaccharides was more than 40% for 0.88 M lactose solution at 70 degrees C at pH 7.0. The major product was a trisaccharide, 3'-galactosyllactose, formed by a galactosyltransfer reaction.

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