Appl Environ Microbiol. 2001 Apr;67(4):1412-7.

Long-range (1)H-(15)N heteronuclear shift correlation at natural abundance: a tool to study benzothiazole biodegradation by two rhodococcus strains.

Besse P, Combourieu B, Boyse G, Sancelme M, De Wever H, Delort AM.

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Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 du CNRS, Université Blaise Pascal, 63177 Aubière Cedex, France.

Abstract

The biodegradation of benzothiazole and 2-hydroxybenzothiazole by two strains of Rhodococcus was monitored by reversed phase high-pressure liquid chromatography and by (1)H nuclear magnetic resonance (NMR). Both xenobiotics were biotransformed into a hydroxylated derivative of 2-hydroxybenzothiazole by these two strains. The chemical structure of this metabolite was determined by a new NMR methodology: long-range (1)H-(15)N heteronuclear shift correlation without any previous (15)N enrichment of the compound. This powerful NMR tool allowed us to assign the metabolite structure to 2,6-dihydroxybenzothiazole.

PMID:: 11282584; [PubMed - indexed for MEDLINE]
PMCID:: PMC92748

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