Abstract
[structure: see text]. Reported here is the synthesis of oligonucleotides containing a 2'-acylamido-2'-deoxyuridine residue at their 3'-terminus. Compared to control sequences bearing a thymidine residue, the quinolone-capped duplexes give higher UV melting points. In the case of (5'-ACGCGU-NA-2')2, where NA denotes a nalidixic acid residue, the melting point increase is up to 22 degrees C over that of (ACGCGT)2.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Anti-Infective Agents / chemistry
-
Base Pairing
-
Deoxyuridine / analogs & derivatives*
-
Deoxyuridine / chemistry
-
Molecular Structure
-
Nalidixic Acid / chemistry*
-
Nucleic Acid Conformation
-
Nucleic Acid Hybridization*
-
Oligonucleotides / chemical synthesis*
-
Oligonucleotides / chemistry
-
Quinolones / chemistry*
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Temperature
-
Ultraviolet Rays
Substances
-
Anti-Infective Agents
-
Oligonucleotides
-
Quinolones
-
Nalidixic Acid
-
Deoxyuridine