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J Bacteriol. 2001 Apr;183(8):2667-71.

Twelve-transmembrane-segment (TMS) version (DeltaTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity.

Author information

  • 1Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.

Abstract

A "Tet(L)-12" version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and antiport with K+ as a coupling ion as well as or better than wild-type Tet(L) but exhibited no tetracycline-Me2+/H+ antiport in Escherichia coli vesicles.

PMID:
11274128
[PubMed - indexed for MEDLINE]
PMCID:
PMC95185
Free PMC Article

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