Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells

Z H Lee; S E Lee; K Kwack; W Yeo; T H Lee; S S Bae; P G Suh; H H Kim

Caspase-mediated cleavage of TRAF3 in FasL-stimulated Jurkat-T cells

J Leukoc Biol. 2001 Mar;69(3):490-6.

Authors

Z H Lee¹, S E Lee, K Kwack, W Yeo, T H Lee, S S Bae, P G Suh, H H Kim

Affiliation

¹ National Research Laboratory for Bone Metabolism and Research Center for Proteineous Materials, Chosun University School of Dentistry, Kwangju, Korea.

PMID: 11261798

Abstract

The tumor necrosis factor receptor (TNFR)-associated factor (TRAF) proteins play a central role in the early steps of signal transduction by TNFR superfamily proteins, which induce various cellular responses, including apoptosis. Influences of TRAF proteins on the regulation of cell death and physical interactions between TRAFs and caspases have been reported. In this study, we demonstrate that TRAF3 is proteolyzed during cell death in a caspase-dependent manner. TRAF3 was found to be cleaved by incubation with caspase3 in vitro and by Fas- or CD3-triggering in Jurkat-T cells. The Fas- or CD3-induced cleavage of TRAF3 was blocked by caspase inhibitors and by introduction of alanine substitutions for D347 and D367 residues. Furthermore, the amino-terminal fragment of TRAF3 showed a different intracellular localization from the full-length TRAF3 with preferential distribution to particulate fractions and the nucleus. These findings suggest that TRAF3 may be regulated by caspases during apoptosis of T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Chloromethyl Ketones / pharmacology
Animals
Apoptosis / drug effects
Apoptosis / physiology
COS Cells / enzymology
COS Cells / metabolism
Caspase 3
Caspase Inhibitors
Caspases / metabolism*
Cysteine Proteinase Inhibitors
Fas Ligand Protein
HeLa Cells
Humans
Jurkat Cells / drug effects
Jurkat Cells / enzymology
Jurkat Cells / metabolism*
Membrane Glycoproteins / pharmacology*
Muromonab-CD3 / pharmacology
Oligopeptides / pharmacology
Proteins / metabolism*
Recombinant Proteins / metabolism
Recombinant Proteins / pharmacology
TNF Receptor-Associated Factor 3

Substances

Amino Acid Chloromethyl Ketones
Caspase Inhibitors
Cysteine Proteinase Inhibitors
FASLG protein, human
Fas Ligand Protein
Membrane Glycoproteins
Muromonab-CD3
Oligopeptides
Proteins
Recombinant Proteins
TNF Receptor-Associated Factor 3
benzoylcarbonyl-aspartyl-glutamyl-valyl-aspartyl-fluoromethyl ketone
benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
CASP3 protein, human
Caspase 3
Caspases