A new ceramide, named tuber-ceramide (2), along with a known cerebroside (1) were isolated from the seeds of Allium tuberosum. The structure of tuber-ceramide was determined on the basis of spectral data as N-(2',3'-dihydroxy-tetracosenoyl)-2-amino-1,3,4-trihydroxy octadecane (2). This is the first report of sphingosine derivatives isolated from the genus Allium.