Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Philos Trans R Soc Lond B Biol Sci. 2001 Feb 28;356(1406):169-76.

Investigating protein conformation-based inheritance and disease in yeast.

Author information

  • 1Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA. s-lindquist@uchicago.edu

Abstract

Our work supports the hypothesis that a protein can serve as an element of genetic inheritance. This protein-only mechanism of inheritance is propagated in much the same way as hypothesized for the transmission of the protein-only infectious agent in the spongiform encephalopathies; hence these protein factors have been called yeast prions. Our work has focused on [PSI(+)], a dominant cytoplasmically inherited factor that alters translational fidelity. This change in translation is produced by a self-perpetuating change in the conformation of the translation-termination factor, Sup35. Most recently, we have determined that new elements of genetic inheritance can be created by deliberate genetic engineering, opening prospects for new methods of manipulating heredity. We have also uncovered evidence that other previously unknown elements of protein-based inheritance are encoded in the yeast genome. Finally, we have begun to use yeast as a model system for studying human protein folding diseases, such as Huntington's disease. Proteins responsible for some of these diseases have properties uncannily similar to those that produce protein-based mechanisms of inheritance.

PMID:
11260797
[PubMed - indexed for MEDLINE]
PMCID:
PMC1088422
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk