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J Chromatogr A. 2001 Jan 12;907(1-2):337-42.

Effect of liquid chromatography separation of complex matrices on liquid chromatography-tandem mass spectrometry signal suppression.

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  • 1Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.


The effect of liquid chromatography separation on liquid chromatography-tandem mass spectrometry (LC-MS-MS) signal response for the characterization of low-molecular-mass compounds in a complex matrix was investigated. Matrix induced signal suppression appears throughout the entire LC-MS-MS analysis of wheat forage extract, with greatest suppression occurring at early retention times. Experimental results show that co-elution of matrix components and analytes from the LC column may be most strongly attributed to column overloading rather than similar analyte and matrix retention behavior. As a result, two-dimensional (LC-LC) separation can be a highly effective approach to address signal suppression effects for the quantitative LC-MS-MS analysis of complex matrix samples.

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