Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Traffic. 2000 Oct;1(10):755-62.

Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells.

Author information

  • 1Department of Cell Biology, BCC 162, Scripps Research Institute, 10550 N Torrey Pines Rd, La Jolla, CA 92037, USA.

Abstract

The advent of GFP imaging has led to a revolution in the study of live cell protein dynamics. Ease of access to fluorescently tagged proteins has led to their widespread application and demonstrated the power of studying protein dynamics in living cells. This has spurred development of next generation approaches enabling not only the visualization of protein movements, but correlation of a protein's dynamics with its changing structural state or ligand binding. Such methods make use of fluorescence resonance energy transfer and dyes that report changes in their environment, and take advantage of new chemistries for site-specific protein labeling.

PMID:
11208065
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Blackwell Publishing
    Loading ...
    Write to the Help Desk