A random amplified polymorphic DNA polymerase chain reaction technique that differentiates between Neospora species

J A Spencer; A K Witherow; B L Blagburn

doi:10.1645/0022-3395(2000)086[1366:ARAPDP]2.0.CO;2

A random amplified polymorphic DNA polymerase chain reaction technique that differentiates between Neospora species

J Parasitol. 2000 Dec;86(6):1366-8. doi: 10.1645/0022-3395(2000)086[1366:ARAPDP]2.0.CO;2.

Authors

J A Spencer¹, A K Witherow, B L Blagburn

Affiliation

¹ Department of Pathobiology, College of Veterinary Medicine, Auburn University, Alabama 36849, USA.

PMID: 11191920
DOI: 10.1645/0022-3395(2000)086[1366:ARAPDP]2.0.CO;2

Abstract

Neospora caninum is a recently described coccidial parasite that was first isolated from a dog in 1988 and has subsequently been shown to infect a wide range of mammals. Neospora hughesi, a new species of this genus, has recently been isolated from the spinal cord of horses showing clinical signs of equine protozoal myeloencephalitis. The random amplified polymorphic DNA polymerase chain reaction technique is capable of differentiating between N. caninum and N. hughesi.

MeSH terms

Animals
Coccidiosis / diagnosis
Coccidiosis / parasitology
Coccidiosis / veterinary*
DNA Primers / chemistry
DNA, Protozoan / analysis
Diagnosis, Differential
Dogs
Encephalomyelitis / diagnosis
Encephalomyelitis / parasitology
Encephalomyelitis / veterinary*
Horse Diseases / diagnosis
Horse Diseases / parasitology*
Horses
Neospora / classification*
Neospora / genetics
Neospora / isolation & purification
Random Amplified Polymorphic DNA Technique / veterinary*
Spinal Cord / parasitology

Substances

DNA Primers
DNA, Protozoan