High-throughput reverse genetics: RNAi screens in ...[Genome Biol. 2001]

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1: Genome Biol. 2001;2(2):REVIEWS1005. Epub 2001 Jan 31. Links

High-throughput reverse genetics: RNAi screens in Caenorhabditis elegans.

Bargmann CI.

Howard Hughes Medical Institute, Departments of Anatomy and of Biochemistry and Biophysics, The University of California, San Francisco, CA 94143, USA. cori@itsa.ucsf.edu

Two recent chromosome-wide screens for phenotypes caused by RNA-mediated interference (RNAi) in Caenorhabditis elegans have increased our understanding of essential genes in nematodes. These papers represent a major advance in functional genomics.

PMID: 11182891 [PubMed - indexed for MEDLINE]

PMCID: PMC138903

New genes with roles in the C. elegans embryo revealed using RNAi of ovary-enriched ORFeome clones.
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[Genome Res. 2005]
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[Methods Mol Biol. 2006]
Genomics: frontiers of gene function.
Nature. 2005 Mar 24; 434(7032):444-5.

[Nature. 2005]
ReviewUncover genetic interactions in Caenorhabditis elegans by RNA interference.
Biosci Rep. 2005 Oct-Dec; 25(5-6):299-307.

[Biosci Rep. 2005]
ReviewHigh-throughput RNAi in Caenorhabditis elegans: genome-wide screens and functional genomics.
Differentiation. 2004 Mar; 72(2-3):81-91.

[Differentiation. 2004]
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Cited by 2 PubMed Central articles

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Kherlopian AR, Song T, Duan Q, Neimark MA, Po MJ, Gohagan JK, Laine AF. BMC Syst Biol. 2008 Aug 12; 2:74. Epub 2008 Aug 12.

[BMC Syst Biol. 2008]
Effects of genetic mutations and chemical exposures on Caenorhabditis elegans feeding: evaluation of a novel, high-throughput screening assay.
Boyd WA, McBride SJ, Freedman JH. PLoS One. 2007 Dec 5; 2(12):e1259. Epub 2007 Dec 5.

[PLoS One. 2007]

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