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    Mol Cell. 2001 Jan;7(1):193-203.

    A conserved HEAT domain within eIF4G directs assembly of the translation initiation machinery.

    Source

    Laboratories of Molecular Biophysics, The Rockefeller University, New York, NY 10021, USA.

    Abstract

    The X-ray structure of the phylogenetically conserved middle portion of human eukaryotic initiation factor (eIF) 4GII has been determined at 2.4 A resolution, revealing a crescent-shaped domain consisting of ten alpha helices arranged as five HEAT repeats. Together with the ATP-dependent RNA helicase eIF4A, this HEAT domain suffices for 48S ribosomal complex formation with a picornaviral RNA internal ribosome entry site (IRES). Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of this essential component of the translation initiation machinery that, respectively, bind eIF4A and a picornaviral IRES. The structural and biochemical results provide mechanistic insights into both cap-dependent and cap-independent translation initiation.

    PMID:
    11172724
    [PubMed - indexed for MEDLINE]

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