Abstract
The cDNA of an uncoupling protein (UCP) homologue was obtained by screening a chicken skeletal-muscle library. The predicted 307-amino-acid sequence of avian UCP (avUCP) is 55, 70, 70 and 46% identical with mammalian UCP1, UCP2 and UCP3 and plant UCP respectively. avUCP mRNA expression is restricted to skeletal muscle and its abundance was increased 1.3-fold in a chicken line showing diet-induced thermogenesis, and 3.6- and 2.6-fold in cold-acclimated and glucagon-treated ducklings developing muscle non-shivering thermogenesis respectively. The present data support the implication of avUCP in avian energy expenditure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Avian Proteins*
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Base Sequence
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Blotting, Northern
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Chickens
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DNA Primers
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DNA, Complementary
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Mitochondrial Proteins*
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Mitochondrial Uncoupling Proteins
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Molecular Sequence Data
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Muscle, Skeletal / physiology*
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Homology, Amino Acid
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Thermogenesis / physiology*
Substances
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Avian Proteins
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Carrier Proteins
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DNA Primers
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DNA, Complementary
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Mitochondrial Proteins
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Mitochondrial Uncoupling Proteins
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RNA, Messenger
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mitochondrial uncoupling protein, chicken