FEBS Lett. 2001 Jan 19;488(3):116-22.

The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins.

Olalla L, Aledo JC, Bannenberg G, Márquez J.

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Departamento de Biología Molecular y Bioquímica, Laboratorio de Química de Proteínas, Facultad de Ciencias, Universidad de Málaga, 29071, Málaga, Spain.

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FEBS Lett 2002 Nov 20;531(3):570.

Abstract

The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate. We used the two-hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, alpha1-syntrophin and a glutaminase-interacting protein, named GIP, that showed association with human glutaminase L, as deduced from specificity test of the two-hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372-base open reading frame encoding a 124-amino acids protein. Glutaminase associates with both PDZ proteins through its C-terminal end; mutagenesis of single amino acids revealed the sequence -ESXV as essential for the interaction. These data suggest the possibility that PDZ domain-containing proteins are involved in the regulation of glutaminase in brain.

PMID:: 11163757; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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GENBANK/AF234997

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