The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit

Cell. 2000 Dec 22;103(7):1143-54. doi: 10.1016/s0092-8674(00)00216-6.

Abstract

We have used the recently determined atomic structure of the 30S ribosomal subunit to determine the structures of its complexes with the antibiotics tetracycline, pactamycin, and hygromycin B. The antibiotics bind to discrete sites on the 30S subunit in a manner consistent with much but not all biochemical data. For each of these antibiotics, interactions with the 30S subunit suggest a mechanism for its effects on ribosome function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / chemistry
  • Binding Sites
  • Crystallography, X-Ray
  • Hygromycin B / pharmacology*
  • Molecular Mimicry
  • Pactamycin / pharmacology*
  • Protein Structure, Tertiary
  • Protein Synthesis Inhibitors / pharmacology*
  • RNA, Messenger / chemistry
  • RNA, Transfer / chemistry
  • Ribosomal Proteins / chemistry
  • Ribosomes / chemistry
  • Ribosomes / drug effects*
  • Structure-Activity Relationship
  • Tetracycline / pharmacology*
  • Thermus thermophilus

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Protein Synthesis Inhibitors
  • RNA, Messenger
  • Ribosomal Proteins
  • Pactamycin
  • Hygromycin B
  • RNA, Transfer
  • Tetracycline

Associated data

  • PDB/1HNW
  • PDB/1HNX
  • PDB/1HNZ